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. 2021 May 18;10(5):797. doi: 10.3390/antiox10050797

Figure 4.

Figure 4

TLR4/MyD88/NFκB signaling pathway and differences in protein levels in hGMSCs. Expression of TLR4, MYD88 and NFκB, examined by confocal microscopy and Western blot experiments. (A1–D1) TLR4 expression in hGMSCs (CTRL), hGMSCs + AA, hGMSCs + LPS-G, hGMSCs + AA/LPS-G. (A2–D2) MyD88 expression in hGMSCs (CTRL), hGMSCs + AA, hGMSCs + LPS-G, hGMSCs + AA/LPS-G. (A3–D3) NFκB expression in hGMSCs (CTRL), hGMSCs + AA, hGMSCs + LPS-G, hGMSCs + AA/LPS-G. (E) TLR4, MyD88 and NFκB protein levels. β-actin was used as a loading control. Green fluorescence: cytoskeleton actin. Red fluorescence: TLR4, MYD88, NFκB. Blue fluorescence: cell nuclei. Scale bar: 20 µm. * p < 0.05; ** p < 0.01; *** p < 0.001.