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. 2021 May 18;10(5):797. doi: 10.3390/antiox10050797

Figure 6.

Figure 6

TLR4/MyD88/NFκB signaling pathway and differences in protein levels in e-hGMSCs. Expression of TLR4, MYD88 and NFκB, examined by confocal microscopy and Western blot experiments. (A1–D1) TLR4 expression in e-hGMSCs (CTRL), e-hGMSCs + AA, e-hGMSCs + LPS-G, e-hGMSCs + AA/LPS-G. (A2–D2) MyD88 expression in e-hGMSCs (CTRL), e-hGMSCs + AA, e-hGMSCs + LPS-G, e-hGMSCs + AA/LPS-G. (A3–D3) NFκB expression in e-hGMSCs (CTRL), e-hGMSCs + AA, e-hGMSCs + LPS-G, e-hGMSCs + AA/LPS-G. (E) TLR4, MyD88 and NFκB protein levels. β-actin was used as a loading control. Green fluorescence: cytoskeleton actin. Red fluorescence: TLR4, MYD88, NFκB. Blue fluorescence: cell nuclei. Scale bar: 20 µm. * p < 0.05; ** p < 0.01.