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The detection of endogenous H2S via LC-MS. (A) The chromatography of SDB, peak 1/SDB, peak 2/hydrocortisone (internal standard); (B) the calibration curve of SDB in different concentration (0.625~20 μM); (C) the 3-day endogenous H2S concentration changes in plasma after a single injection of SPRC or SPRC@PLA. Dosage of 100 mg kg−1 was calculated according to rats’ body weight (n = 3, mean ± SD).
