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. 2021 May 26;12:300. doi: 10.1186/s13287-021-02375-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Epothilone D promotes NSC differentiation into neurons. a Epothilone D promoted the neuronal differentiation of spinal cord NSCs and inhibited their differentiation into astrocytes. Scale bars, 50 μm. b Quantification of percentages of TUJ1(+) cells among differentiated cells cultured with or without Epothilone D. c Quantification of percentages of GFAP(+) cells among differentiated cells cultured with or without Epothilone D. d, e Tuj1 and NeuN mRNA levels in lysates of differentiated cells were analyzed by qRT-PCR after Epothilone D induction. f, g Western blot detection of the effect of Epothilone D on neuronal differentiation of NSCs. Error bars represent the mean ± SD