Fig. 2.

Transplantation of NSCs exposed to Epothilone D can promote neural circuit formation after SCI. a Pseudotyped rabies virus-mediated monosynaptic retrograde tracing was used to map the synaptic inputs to newborn neurons in the spinal cord. Transplantation of NSCs induced with Epothilone D promoted the formation of neural circuits in the injured site. Newborn cells (both green and red) in the injury site (T8) and traced cells (red only) in the normal spinal cord area (cervical section). Traced cells in the cervical section expressed NeuN protein. Scale bars are 500 μm or 50 μm. b The ratio of traced/starter cells was analyzed in the cervical section in a. c The ratio of traced/starter cells was analyzed in the injured site in a. d Diagram of the in vivo function patterns of Epothilone D before RNA-Seq. e, f RNA-Seq results revealed differentially expressed genes. g qRT-PCR analysis was used to detect upregulated differentially expressed gene during the differentiation of NSCs in vitro. Error bars represent the mean ± SD