Table 3.
Hydrogen peroxide stress tolerance for strains with and without β-caryophyllene production after 200 mM H2O2 exposure for 30 min
| Mutation | No production | With production | |||
|---|---|---|---|---|---|
| Strain | Relative H2O2 tolerance | Strain | Relative H2O2 tolerance | β-caryophyllene (mg/g DCW) | |
| Control | YAG114 | YAG118 | 3.39 | ||
| CDC39 | YAG119 | ND | YAG132 | ND | 3.55 |
| DAN4 T200T | YAG120 | ND | YAG133 | ND | 3.47 |
| FLO9 T288T | YAG121 | + | YAG134 | + | 3.15 |
| tK(CUU)C/MAK32 int | YAG122 | ND | YAG135 | ND | 3.44 |
| MDS3 | YAG123 | + | YAG136 | + | 2.94 |
| MST27/tR(UCU)G1 int | YAG124 | + | YAG137 | + | 10.27 |
| MTL1 S257S | YAG125 | + | YAG138 | + | 2.24 |
| RIF1 D709N | YAG126 | ND | YAG139 | ND | 3.36 |
| RNR3/FIS1 int | YAG127 | ND | YAG140 | ND | 3.31 |
| SNO2/SNZ2 int | YAG128 | ND | YAG141 | ND | 3.57 |
| STE6 T1025N | YAG129 | ND | YAG142 | + | 12.66 |
| YAL064W-B/TDA8 int | YAG130 | ND | YAG143 | ND | 3.35 |
| YHL050C int | YAG131 | ND | YAG144 | ND | 3.25 |
Production was measured in 20 mL test tube with 3 mL culture overlayed with 500 μL dodecane. ND: not different from wild-type. +: ~ 10× higher survival compared to wild-type. Bold: p value < 0.05 using two-tailed Student’s t test compared with control