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. Author manuscript; available in PMC: 2021 May 27.
Published in final edited form as: J Vis Exp. 2019 May 24;(147):10.3791/59177. doi: 10.3791/59177

Figure 1: Exosome quantification scheme.

Figure 1:

(A) Schematic of multi-well protein A/G slides (192 wells) used in this assay. (B) Target exosomes are directly captured from samples, including serum and plasma, by surface immobilization on the capture antibody (e.g. anti-EphA2 antibody) bound to the slide, and then incubated with gold nanorods conjugated with a detection antibody (e.g. anti-CD9 antibody) before analysis by DFM image analysis.