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. 2021 May 18;11(5):451. doi: 10.3390/life11050451

Figure 4.

Figure 4

Expression of Wnt/β-catenin/GSK3β pathway under both dose- and fraction-dependent manners in irradiated U87MG and GBM8401 cells. (A) GBM8401 cells were treated with radiation for 72 h, and the protein expression patterns of the Wnt pathway were determined. Phospho-β-catenin (Ser33/Ser37/Thr41) was used as β-catenin active forms for detecting the β-catenin status. GSK3β (S9) was used for β-catenin phosphorylation to degrade β-catenin. (B) The cells were irradiated with indicated dosages and were then incubated with MG-132 (10 µM) for 8 h. Cytosolic fractions were prepared and subjected to Western blotting with frizzled, GSK3β, phosphor S9 GSK3β, β-catenin, phosphor S33/37/41-β-catenin, and P62 antibodies. GAPDH was used as an internal control. Bar graphs represent the mean of triplicates ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the control group.