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. 2021 May 18;10(5):798. doi: 10.3390/antiox10050798

Figure 2.

Figure 2

Figure 2

Inhibition of CAPE and its analogues on the formation of γ-H2AX induced by iron-overload in HeLa cells, as measured by immunofluorescence staining. (A) Representative images of HeLa cells stained for Hoechst 33342 (nuclear stain) monoclonal antibodies of γ-H2AX under different conditions as indicated. (B) Quantitation of γ-H2AX formation in HeLa cells under different conditions as indicated. *** Significant difference from the Fe(II)–H2O2 groups, p < 0.001. The experiment was repeated three times, and thirty cells were randomly selected in different microscopic fields for statistical analysis in each group.