Figure 6.
UV spectral changes observed upon the addition of Fe(III)–citrate to CAPE and its analogues. (A) the incremental addition of Fe(III)–citrate (25–400 μM) to 100 μM CAPE decreased the absorbance peak at 322 nm, with concomitant increases at 350 and 570 nm. (B) the incremental addition of Fe(III)–citrate (25–400 μM) to 100 μM CAPE generated new absorbance peaks at 372 and 610 nm. (C,D) No significant new absorbance peak was observed in FA and EF with Fe(III)–citrate. The reaction mixture contained 100 μM CAPE or its analogues and different concentrations of Fe(III)–citrate (25–400 μM). The experiments were repeated three times, and only typical spectra are presented.