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. 2021 May 18;13(10):2438. doi: 10.3390/cancers13102438

Figure 5.

Figure 5

Loss of Pgrmc1 suppresses IL-6 production in macrophages. (A,B) Plasma level of IL-6 (A) and hepatic mRNA levels of IL-1α, IL-1β, and Tnf (B) in DEN-injected WT and Pgrmc1-null mice. The mRNA expression levels were normalized to that of Rplp0 and expressed relative to the WT group. * p < 0.05 in Student’s t-test. (C,D) Culture supernatant level of IL-6 (C) and mRNA expression levels of IL-1α, IL-1β, and Tnf (D) in Raw 264.7 cells transfected with Control (Ctrl) or Pgrmc1-siRNA (KD) and treated with or without necrotic debris for 3 h. The mRNA expression levels were normalized to that of Rplp0 and expressed relative to the Ctrl group. * p < 0.05 in one-way ANOVA followed by Tukey’s post hoc test. (E,F) Control (Ctrl) or Pgrmc1-siRNA (KD)-transfected cells were treated with erlotinib (50 μM) and necrotic debris for 8 h. Level of IL-6 (E) and mRNA expression levels of IL-1α, IL-1β, and Tnf (F) in the supernatant of erlotinib-treated Raw 264.7 cells. The mRNA expression levels were normalized to that of Rplp0 and expressed relative to the Ctrl group. * p < 0.05 in one-way ANOVA followed by Tukey’s post hoc test.