TABLE 1.
Biliary Lipid Composition of Gallbladder and Hepatic Bile
| Mice | G-1 (μg/day) | Ch (Mole%) | PL (Mole%) | BS (Mole%) | Ch/PL Ratio | Ch/BS Ratio | [TL] (g/dL) | CSI |
|---|---|---|---|---|---|---|---|---|
| Pooled gallbladder bile* | ||||||||
| Wild-type | 0 | 6.14 | 15.85 | 78.01 | 0.39 | 0.08 | 8.93 | 1.10 |
| Wild-type | 1 | 9.95 | 18.03 | 72.02 | 0.55 | 0.14 | 9.81 | 1.55 |
| GPR30−/− | 1 | 5.03 | 13.01 | 81.96 | 0.39 | 0.06 | 9.65 | 1.02 |
| ERα−/− | 1 | 8.71 | 16.77 | 74.52 | 0.52 | 0.12 | 10.15 | 1.43 |
| Individual hepatic bile* | ||||||||
| Wild-type | 0 | 6.38 ± 0.69 | 19.20 ± 1.04 | 74.42 ± 1.19 | 0.33 ± 0.04 | 0.09 ± 0.01 | 2.31 ± 0.38 | 1.31 ± 0.14 |
| Wild-type | 1 | 7.57 ± 1.53 | 22.07 ± 4.22 | 70.36 ± 5.21 | 0.35 ± 0.07 | 0.11 ± 0.03 | 1.57 ± 0.17 | 1.55 ± 0.28 |
| GPR30−/− | 1 | 4.36 ± 0.76 | 14.59 ± 3.13 | 81.06 ± 3.83† | 0.30 ± 0.03 | 0.05 ± 0.01‡ | 2.05 ± 0.45 | 1.17 ± 0.08† |
| ERα−/− | 1 | 7.29 ± 0.26 | 19.59 ± 1.66 | 73.12 ± 1.58 | 0.37 ± 0.04 | 0.10 ± 0.00 | 1.16 ± 0.19 | 1.75 ± 0.11 |
*
Values were determined from pooled gallbladder bile (n = 10 per group) and from individual gallbladder bile (n = 5 per group).
†
P < 0.001 compared to wild-type mice treated with G-1 at 1 μg/day and fed the lithogenic diet for 8 weeks.
‡
P < 0.05 compared to wild-type mice treated with G-1 at 1 μg/day and fed the lithogenic diet for 8 weeks.
Abbreviations: [TL], total lipid concentration; BS, bile salts; Ch, cholesterol; PL, phospholipids.