Fig. 7.

Validation of expression levels of EDNRB, RXFP1, P2RY1, CALCRL, TEK, and ANGPT1 in LAC patients. To further validate the expression level in LAC patients, six genes were re-analyzed via real-time PCR (a) and immunohistochemistry (b) analysis. Representative images of IHC staining were shown. Scale bar, 50 μm. Real-time PCR data were presented as mean ± SEM and the differences were estimated by Wilcoxon paired signed-rank test (*P < 0.05, **P < 0.01, ***P < 0.001). Data were normalized to GAPDH expression. All six genes were markedly weakly expressed in LAC tissue compared to adjacent normal tissue