Fig. 3. (A) Secondary structure of the Res-A10-Right-cut. (B) Comparison of specificity of the Res-A10-Right-cut and the wild-type aptamer. 50 nM DNA mixed with 2 mM testing molecules in buffer A (20 mM HEPES, 100 mM NaCl, and 5 mM MgCl2) with 50 nM SGI. (C) Titrating adenosine or AMP into 50 nM Res-A10-Right-cut in buffer A. (D) Secondary structure of the Res-A10-Left-cut. (E) Comparison of specificity of the Res-A10-Left-cut and wild-type aptamer. (F) Titrating adenosine or AMP (40 μM to 2 mM) into the Res-A10-Left-cut.
