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. 2021 May 27;20:79. doi: 10.1186/s12943-021-01377-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression and prognostic significance of EYA2 in HCC tissues. (A) EYA2 expression in HCC and adjacent tissues according to the three datasets GES22058, GSE14520 and ICGC_LIRI. (B) Western blot analysis of EYA2 expression in HCC and adjacent tissues. T: HCC tissues, N: Adjacent tissues. (C) A representative immunohistochemical staining of EYA2 in HCC and case-matched adjacent tissue. (D) Immunohistochemistry scores associated to the EYA2 expression in HCC and case-matched adjacent tissues (n = 94 pairs). Kaplan-Meier analysis of overall survival (E) and recurrence-free survival (F) of HCC patients with high or low EYA2 expression (n = 94 cases). **P < 0.01, ***P < 0.001. (A, B, D) mean ± SEM, Student’s t-tests; (E, F) Kaplan-Meier analysis