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. 2021 May 27;20:79. doi: 10.1186/s12943-021-01377-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — EYA2 suppresses the malignant phenotypes of HCC cells in vitro and in vivo. (A) Western blot analysis of the expression of EYA2 in HCC cells transiently transfected with siRNA or with the overexpression and mutant vectors. The effect of siRNA-mediated knockdown of EYA2 and pcDNA3.1-mediated overexpression of constructs encoding EYA2 wild-type, EYA2(R255K) and EYA2(A510E) on cell proliferation (B), cell invasion (C), clone formation (D) and cell migration (E) of HCC cells in vitro. (F) The effect of EYA2 knockdown, EYA2 wild-type and EYA2(A510E) mutant on HCC tumor growth in nude mice. (G) The scheme of treatment of orthotopic HCC tumors in nude mice by lentivirus-EYA2. (H) Images, volume and weight of the orthotopic liver tumors of the mice. (I) Expression of EYA2 in tumor tissues detected by immunohistochemistry analysis. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001. (B–F, H–I) mean ± SEM, Student’s t-tests