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. Author manuscript; available in PMC: 2021 May 27.
Published in final edited form as: J Cell Physiol. 2013 Sep;228(9):1819–1826. doi: 10.1002/jcp.24333

Fig. 1.

Fig. 1.

A, B: Voiding behavior analysis using the VSOP (Voided Stain On the Paper) method showed increase in bladder capacity (A) and decrease in urinary frequency (B) in 15-week-old mice compared to 3-week-old mice. C: Bladder contractility examination with bladder muscle strips showed same normal sensitivity to cholinergic agents in both 3-week-old mice and 15-week-old mice. D: qPCR data showed myocardin (MYOCD) and SM markers were down-regulated in 15-week-old mice compared to 3-week-old mice. E: Western blotting also revealed that SM markers were down-regulated in 15-week-old mice compared to 3-week-old mice. F: Immunohsitochemistry showed that ACTA2 was down-regulated in bladders of 15-week-old mice compared to those of 3-week-old mice. *Statistically significant difference (P < 0.05).