TABLE 1.
No. | Gene | Primer sequence | Application | Size (bp) | |
---|---|---|---|---|---|
1 | Myocd (mouse) | For | AAGGTCCATTCCAACTGCTC | qPCR | 216 |
Rev | CCATCTCTACTGCTGTCATCC | ||||
2 | Acta2 (mouse) | For | GCTTCGCTGGTGATGATGCTC | qPCR | 177 |
Rev | AGTTGGTGATGATGCCGTGTTC | ||||
3 | Cnn1 (mouse) | For | GGACCAGGCGACCATCAG | qPCR | 297 |
Rev | TAGGCAGAGTTGTAGTAGTTGTG | ||||
4 | Myh 11 (mouse) | For | TGCCGACACAGCCTACAGAAG | qPCR | 130 |
Rev | GGACGCCACCACAGCCAAG | ||||
5 | Gja1 (mouse) | For | AACAGCAGCAGACTTTGAAAC | qPCR | 234 |
Rev | AATGAAGAGCACCGACAGC | ||||
6 | Gapd (mouse) | For | CGTGCCGCCTGGAGAAAC | RT-PCR and qPCR | 136 |
Rev | TGGGAGTTGCTGTTGAAGTCG | ||||
7 | Myocd (mouse) | For | TTCTGCCGATGGATTCTTCCGTGA | RT-PCR | 208 |
Rev | AGATCCTGGATTGTGTCCAGAGGA | ||||
8 | Cnn1 (rat) | For | ACACTTTAACCGAGGTCCTGCCTA | qPCR | 154 |
Rev | CTTGAGGCCATCCATGAAGTTGCT | ||||
9 | Acta2 (rat) | For | GCTGTGCTATGTCGCTCTGG | qPCR | 157 |
Rev | AATGAAAGATGGCTGGAAGAGG | ||||
10 | Krt20 (rat) | For | ATGCGGATAACTGTGGAAGC | qPCR | 187 |
Rev | CCTCCACGTTGACATTGTTG | ||||
11 | Myocd (rat) | For | CAGAAAGTGACAAGAACGATACAG | qPCR | 265 |
Rev | TGAAGCAGCCGAGCATAGG | ||||
12 | Gja1 (rat) | For | GAAAGAGAGGTGCCCAGAC | qPCR | 199 |
Rev | GCCAGGTTGTTGAGTGTTAC | ||||
13 | Gapd (rat) | For | GCAAGTTCAACGGCACAGTCAAG | qPCR | 124 |
Rev | ACATACTCAGCACCAGCATCACC |
Each number (No.) indicates the pair of primers used to amplify the gene listed.