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Fluorescence microscopic images (overlay of bright field and fluorescence channels) of untreated TR146 cells (A) and cells treated with 400 ng/mL IL-1ß (B) and 300 ng/mL TNF-α (C). Circles indicate specific binding of Annexin V-Alexa Fluor 488 (green) to PS on the outside of the cells. Arrows show cell blebbing (green spots) and stars indicate PI stained necrotic cells (red).
