Table III.
Results of rpoB gene sequencing in five discordant samples at All India Institute of Medical Sciences, New Delhi
| Truenat MTB-RIF result | GeneXpert MTB/RIF result | Reported MIC μg/ml19 | Sanger sequencing rpoB gene mutation (Escherichia coli numbering system) | Treatment given (based on) | GenBank accession number |
|---|---|---|---|---|---|
| Mtb detected; susceptible | Mtb detected; resistant | 32 | Serine 531 leucine | MDR (GeneXpert result) | MT437290 |
| Mtb detected; susceptible | Mtb detected; resistant | 32 | Serine 531 leucine | MDR (GeneXpert result) | MT437291 |
| Mtb detected; resistant | Mtb detected; susceptible | 32 | 518 deletion of asparagine | MDR (clinical presentation) | MT437292 |
| Mtb detected; resistant | Mtb detected; susceptible | 32 | 518 deletion of asparagine | MDR (clinical presentation) | MT437293 |
| Mtb detected; resistant | Mtb detected; susceptible | 32 | 518 deletion of asparagine | MDR (clinical presentation) | MT437294 |
Both the mutations reported here lead to clinical RIF resistance (>1 μg/ml). MDR, multidrug-resistant; Mtb, Mycobacterium tuberculosis; MIC, minimum inhibitory concentration