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. 2021 May 10;118(20):e2021063118. doi: 10.1073/pnas.2021063118

Fig. 4.

Fig. 4.

GABAergic synapse-mediated innate immunity is independent on PMK-1 pathway. (A–C) Survival of wild-type, unc-49(e407), pmk-1(km25), and unc-49(e407);pmk-1(km25) (A) or wild-type, unc-49(e407), nsy-1(ag3), and unc-49(e407);nsy-1(ag3) (B) or wild-type, unc-49(e407), sek-1(km4), and unc-49(e407);sek-1(km4) (C) animals exposed to PA14. The exact P values of statistics for all survival assays are listed in SI Appendix, Table S1. (D) qRT-PCR analyses of the expression levels of six PMK-1–dependent genes in wild-type and unc-49(e407) worms fed on OP50 or exposed to PA14. The exact P values of statistics for comparison of qRT-PCR between groups are listed in SI Appendix, Table S2. (E) Representative image and quantitative analysis of the phosphorylation level of PMK-1 in wild-type and unc-49(e407) mutant animals, respectively. Red star indicates nonspecific band. Data are presented as means ± SEM for D and E. Three independent experiments were performed. Statistical significance was determined by log-rank test for survival assays or one-way ANOVA tests followed by Bonferroni’s multiple comparison tests (D) or unpaired Student’s t test (E). *P < 0.05, **P < 0.01, ***P < 0.001; n.s., not significant.