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Preference for Glu at position -4 relative to the di-leucine of 1105ERAQLL contributes to LB targeting by AP-3. (A) Representative confocal images of MLE15/WT cells expressing mCherry-ABCA3, and WT GFP-ATP8A1, GFP-ATP8A1-AA2, GFP-ATP8A1-E1105D, or GFP-ATP8A1-Q1108A, and immunostained for endogenous RAB11A. (Scale bar, 10 μm.) (B) Pearson’s correlation coefficient acquired from images described in A (n = 2; 15 to 20 cells per group, mean ± SD).
