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. 2021 May 14;118(20):e2025208118. doi: 10.1073/pnas.2025208118

Fig. 6.

Fig. 6.

GFP-ATP8A1 and AP-3 are closely associated with LB-like organelles in MLE15/WT cells. (A) Representative confocal images of proximity ligation assays using MLE15/WT or MLE15/ΔAP3 cells expressing exogenous GFP-ATP8A1-WT or GFP-ATP8A1-AA2. (Scale bar, 10 μm.) Arrowheads identify cytosolic PLA reaction products, and arrows indicate PLA products adjacent to organelles expressing GFP-ATP8A1-WT or GFP-ATP8A1-AA2. Cells were also transfected to express untagged ABCA3 thereby enhancing LB volume for identification. (B) Quantification of AlexaFluor594-positive PLA reaction products in cells described in Fig. 5A (n = 2; 15 to 20 cells; mean ± SD).