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. 2021 May 10;118(20):e2022599118. doi: 10.1073/pnas.2022599118

Fig. 2.

Fig. 2.

INX-1 is a gap junction protein that functionally couples AVL and DVB motor neurons at NMJs. (A) Representative images of AVL/DVB NMJs (arrowhead) and DVB soma (arrow) from young adults coexpressing INX-1::GFP and UNC-10/RIM1::mCherry fusion proteins in GABAergic motor neurons (AVL and DVB) under the unc-47 promoter. (B) Representative images of AVL/DVB NMJs (arrowhead) and DVB soma (arrow) from young adults coexpressing INX-1::GFP in AVL (under the unc-25(Δ) promoter) and INX-1::mCherry in DVB (under the flp-10 promoter). (C) Representative images of AVL/DVB NMJs (arrowhead) and DVB somas (arrow) from young adults coexpressing INX-1::GFP and mouse Cx36::mCherry fusion proteins under the unc-47 promoter. (D) Quantification of the number of Exp per defecation cycle in adults with the indicated genotypes. unc-33c cDNA was expressed under the GABAergic neuron-specific (Punc-47) promoter. “DVB HisCl” denotes expressing HisCl1 under the flp-10 promotor. (E) Quantification of the number of Exp per defecation cycle in adults with the indicated genotypes. Human PANX1 cDNA and synthetic/mouse Cx36 gene were expressed under the GABAergic neuron-specific (Punc-47) promoter. (Scale bar, 10 μm.) Asterisks indicate significant differences: ***P < 0.001 and *P < 0.05 in Student’s t test; n.s., not significant.