Skip to main content
. 2021 May 10;118(20):e2022599118. doi: 10.1073/pnas.2022599118

Fig. 6.

Fig. 6.

Ectopic activation of the DVB motor neuron in inx-1 mutants are suppressed in egl-19 or egl-36(gf) mutants. (A) Diagram of the pacemaker controlled signaling pathway that leads to calcium influx at AVL/DVB NMJs and contraction of the enteric muscle (Exp). NP = neuropeptide; ACY = adenylate cyclase. (B) Quantification of the number of Exp per defecation cycle in adults of the indicated genotypes. PKA[DN] denotes a dominant negative PKA transgene expressed in GABAergic neurons (under the unc-47 promoter). (C) Average frequency of normally timed and ectopic (greater than 5 s) calcium spike per cycle in the indicated mutants. (D and E) Quantification of the number of Exp per defecation cycle in adults of the indicated genotypes. PKA[CA] denotes a constitutively active PKA transgene expressed in GABAergic neurons (under the unc-47 promoter). Neuronal egl-36(gf) denotes egl-36 (gain-of-function) transgene expressed under the GABAergic unc-47 promoter. (F) Average frequency of normally timed and ectopic (greater than 5 s) calcium spike per cycle in the indicated mutants. Means and SEs are shown. Asterisks indicate significant differences: ***P < 0.001 and **P < 0.01 in Student’s t test; n.s., not significant.