Polymorphic sites detected from nonreference sequences in five breeds. (A) Sharing of 83,250 variants across five taurine cattle breeds (BSW: Brown Swiss, HER: Hereford, HOL: Holstein, OBV: Original Braunvieh, SIM: Simmental). (B and C) The top three principal components (PC) of a genomic relationship matrix constructed from nonreference sequence variants separate the animals by breed.