Fig. 4.

Incorrect clock state causes ongoing replication events to abort in the dark. (A, Left) log-survival functions of β-EGFP foci lifetime under various conditions. Solid lines are linear fits through the origin, the negative slopes of which correspond to the apparent dissociation rate constants $\left({\lambda }_{\text{app}\_\text{diss}}\right)$ (Right). Error bars are SEMs bootstrapped from over 2,000 foci trajectories from two to three independent experiments. **P < 0.01 (two-sample t test). (B) SSB-EGFP density time trace in the dark. Error bars are SEMs from two independent experiments. (C) qPCR quantification of ter/oriC ratios in the dark. Error bars are SEMs from four independent experiments. Inset shows ter/oriC ratios averaged throughout the night. ***P < 0.001. (D) Model-predicted ter/oriC ratios assuming 3 to 6 chromosomes per cell (21). (E) dNTP concentrations at subjective dawn and subjective dusk. Error bars are SEMs from four independent experiments. *P < 0.05, **P < 0.01. (F) Fluorescence images showing β-EGFP foci in GalP-expressing cells subject to an out-of-phase dark pulse, without (−) or with (+) glucose supplement. (G) β-EGFP density and apparent dissociation rate constants for GalP-expressing cells. Error bars are SEMs from two (−glucose) or three (+glucose) experiments. (H) Schematic diagram summarizing main findings of this study.