Characterization of egg-BBEA. A, ICC measured among duplicates and averaged across 58 peptides shows high agreement (ICC >0.75) for all antibody isotypes, presented as mean and SD. B, CV averaged across 58 peptides shows low variability among technical replicates, presented as mean and SD C, Unsupervised clustering presented as a phylogenetic tree of the technical duplicates constructed with the Pearson correlation as distance metric and “average” agglomeration algorithm shows that samples from the same patients cluster together