Figure 1.

ELISPOT assay showed the IFNγ secretion of expanded TILs induced by CIR A02:01 cells loaded with in vitro transcribed RNA. TILs derived from patient B2 or B8 were co-cultured with neoantigens-pulsed CIR A02:01 for ten days, and IFNγ secretions of which were detected by ELISPOT. Non-pulsed (NP) control result of B8 was shown as A1-A3; the result of minigene B8-1 pulsed C1R A02:01 cells as A4-A6; B8-2 as B1-B3; B8-3 as B4-B6; B2-1 as C1-C3; B2-2 as C4-C6; non-pulsed of B2 as D1-D3; B2-3 as D4-D6; positive controls of B8 and B2, which were treated with PMA and Ionomycin, were presented as F1-F3 and F4-F6, respectively. Experiments were conducted in triplicate. TNTC represents too numerous to count