Table 1.
List of peptides expressed by minigenes
| mutated Peptide |
Wild-type peptide |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Minigene | gene | Amino acid substitution | Sequence | Affinity to HLA-A (IC50 nM) | Sequence | Affinity to HLA-A (IC50 nM) | tumor_var (RNA) |
mutation ratio |
HLA Alleles |
| B2-1 | AASDH | S589F | FLNSGGDFL | 21 | FLNSGGDSL | 48 | 4 | 27% | A*02: 01 |
| ABCA2 | S715F | MVIFWVYSV | 3 | MVISWVYSV | 3 | 16 | 24% | A*02: 01 | |
| ACOT8 | M252L | FLVSLDHSM | 25 | FMVSLDHSM | 19 | 27 | 25% | A*02: 01 | |
| ACP2 | F393L | LLLIVLLLTV | 23 | FLLIVLLLTV | 10 | 49 | 26% | A*02: 01 | |
| ADCY9 | S938F | YVFLCPDSSV | 39 | YVSLCPDSSV | 182 | 7 | 19% | A*02: 01 | |
| AKR1C3 | P119S | YLIHSSMSL | 4 | YLIHSPMSL | 6 | 23 | 13% | A*02: 01 | |
| AKT2 | S34F | FLLKSDGFFI | 9 | FLLKSDGSFI | 28 | 46 | 10% | A*02: 01 | |
| ALKBH1 | P354S | VLATDQNFSL | 42 | VLATDQNFPL | 25 | 12 | 18% | A*02: 01 | |
| ALMS1 | R4120W | WIYEQLPEV | 4 | RIYEQLPEV | 5 | 6 | 15% | A*02: 01 | |
| ARID1B | S2118 F | FMALLSNLA | 42 | SMALLSNLA | 341 | 34 | 26% | A*02: 01 | |
| ATG2B | E1609K | FLMKIQLSKV | 6 | FLMEIQLSKV | 4 | 7 | 12% | A*02: 01 | |
| ATP6V0A2 | R755K | KLWALSLAHA | 30 | RLWALSLAHA | 47 | 22 | 20% | A*02: 01 | |
| ATXN10 | L159F | FLIITDFFL | 8 | FLIITDLFL | 25 | 54 | 15% | A*02: 01 | |
| BCAR1 | R255Q | ALAQLQQGV | 47 | ALARLQQGV | 74 | 119 | 28% | A*02: 01 | |
| C11orf74 | P63S | SISSCIPFV | 5 | SIPSCIPFV | 8 | 16 | 30% | A*02: 01 | |
| CDCA2 | P815L | KLMESSSVV | 5 | KPMESSSVV | 7200 | 6 | 20% | A*02: 01 | |
| CEPT1 | Q199R | FMFYCAHWRT | 13 | FMFYCAHWQT | 13 | 13 | 15% | A*02: 01 | |
| CHMP2B | E132K | VLDEIGIKI | 47 | VLDEIGIEI | 17 | 35 | 17% | A*02: 01 | |
| B2-2 | CHST2 | P150L | GMAGVAAPL | 21 | GMAGVAAPP | 10959 | 29 | 32% | A*02: 01 |
| CLMP | S2F | FLLLLLLLV | 9 | SLLLLLLLV | 30 | 1 | 30% | A*02: 01 | |
| COL12A1 | T1055R | YQIGWDRFCV | 16 | YQIGWDTFCV | 6 | 17 | 21% | A*02: 01 | |
| COPZ1 | G97R | ALLENMERL | 21 | ALLENMEGL | 15 | 102 | 14% | A*02: 01 | |
| CRYBG3 | A2862T | SLADTRTTSV | 28 | SLADTRATSV | 49 | 1 | 27% | A*02: 01 | |
| CTNS | P73S | TILELSDEV | 24 | TILELPDEV | 36 | 2 | 25% | A*02: 01 | |
| CYP2R1 | E212K | KLFSENVEL | 8 | ELFSENVEL | 277 | 7 | 23% | A*02: 01 | |
| DLST | P25S | SLGRRSLPGV | 49 | PLGRRSLPGV | 4021 | 71 | 28% | A*02: 01 | |
| DNPH1 | E159K | ALLDRYFKA | 10 | ALLDRYFEA | 5 | 39 | 20% | A*02: 01 | |
| DOHH | E208K | ALFRHKVGYV | 38 | ALFRHEVGYV | 22 | 26 | 33% | A*02: 01 | |
| DPY19L3 | V424I | FILSITVIV | 7 | FVLSITVIV | 10 | 7 | 11% | A*02: 01 | |
| DSP | S2077F | SLQDAVFQGV | 18 | SLQDAVSQGV | 37 | 1349 | 14% | A*02: 01 | |
| ECE1 | R524Q | MQFFNFSWRV | 15 | MRFFNFSWRV | 960 | 39 | 15% | A*02: 01 | |
| EPB41L1 | P115L | FAFTVKFYL | 26 | FAFTVKFYP | 8426 | 21 | 16% | A*02: 01 | |
| FAM171B | P645S | VMTSFSSEL | 28 | VMTPFSSEL | 33 | 1 | 23% | A*02: 01 | |
| FANCG | P243L | GLCPRLVLV | 48 | GLCPRPVLV | 119 | 4 | 34% | A*02: 01 | |
| FARS2 | S368L | VINDILFWL | 12 | VINDISFWL | 19 | 61 | 16% | A*02: 01 | |
| FAT1 | P2085L | LLYYAVVKV | 13 | LPYYAVVKV | 11303 | 52 | 12% | A*02: 01 | |
| FURIN | M434I | GLLDAGAIV | 21 | GLLDAGAMV | 13 | 85 | 19% | A*02: 01 | |
| B2-3 | FZD6 | S238F | IIYYFVCYSI | 28 | IIYYSVCYSI | 47 | 9 | 12% | A*02: 01 |
| GAK | R578K | ILVKAVVMTPV | 24 | ILVRAVVMTPV | 33 | 10 | 35% | A*02: 01 | |
| HSPH1 | S393F | FVTDAVPFPI | 19 | SVTDAVPFPI | 140 | 123 | 20% | A*02: 01 | |
| KLF4 | P195L | SLSGGFVAEL | 31 | SPSGGFVAEL | 16501 | 97 | 20% | A*02: 01 | |
| LTN1 | K1279N | FLNYCSSPL | 6 | FLKYCSSPL | 35 | 14 | 18% | A*02: 01 | |
| MAP4K1 | S108F | FLSELQISYV | 4 | SLSELQISYV | 8 | 2 | 18% | A*02: 01 | |
| MED13 | P1679L | FLEMVQTLPL | 29 | FLEMVQTLPP | 4077 | 23 | 23% | A*02: 01 | |
| MED14 | P1344L | VQFCLTIPL | 19 | VQFCLTIPP | 7241 | 25 | 39% | A*02: 01 | |
| MFSD10 | P199L | LLLEMAPWFA | 6 | LPLEMAPWFA | 5755 | 85 | 21% | A*02: 01 | |
| MLXIP | P270L | LLLDTDMLM | 34 | PLLDTDMLM | 3213 | 5 | 19% | A*02: 01 | |
| MRGBP | I111N | VLPEENIQEV | 44 | VLPEEIIQEV | 24 | 72 | 18% | A*02: 01 | |
| MTOR | P896S | ALDSYKHKV | 28 | ALDPYKHKV | 33 | 4 | 18% | A*02: 01 | |
| MYCBP2 | S4018F | YCFELLFMV | 8 | YCFELLSMV | 34 | 24 | 13% | A*02: 01 | |
| MYCBPAP | E606K | VLQKLLMGV | 20 | VLQELLMGV | 8 | 1 | 15% | A*02: 01 | |
| MYO1B | M14I | SLLDNIIGV | 3 | SLLDNMIGV | 3 | 66 | 22% | A*02: 01 | |
| NCOA6 | P657S | LMSQGQMMV | 27 | LMPQGQMMV | 43 | 2 | 21% | A*02: 01 | |
| NRBP2 | F365L | SLMELDKFL | 13 | SFMELDKFL | 6739 | 35 | 15% | A*02: 01 | |
| NUMB | P40L | FLVKYLGHV | 10 | FPVKYLGHV | 6584 | 15 | 15% | A*02: 01 | |
| NVL | S19F | HMNSSLLFL | 28 | HMNSSLLSL | 57 | 8 | 27% | A*02: 01 | |
| B8-1 | AMPD2 | E494K | KLHLFLEHV | 39 | ELHLFLEHV | 1271 | 5 | 25% | A*02: 01 |
| ATN1 | P356S | TLAPSSHSL | 36 | TLAPSPHSL | 50 | 16 | 33% | A*02: 01 | |
| BEND3 | P583L | LLVHLFLEL | 28 | LLVHLFPEL | 11 | 2 | 16% | A*02: 01 | |
| BRPF1 | F292Y | ILYCDMCNL | 21 | ILFCDMCNL | 16 | 8 | 22% | A*02: 01 | |
| C14orf93 | L303F | FVLSKLVHNV | 27 | LVLSKLVHNV | 137 | 8 | 28% | A*02: 01 | |
| C17orf58 | P8L | TLDGFFFRV | 4 | TPDGFFFRV | 8749 | 13 | 21% | A*02: 01 | |
| CAD | S1928Y | SLVGQHILYV | 14 | SLVGQHILSV | 28 | 9 | 19% | A*02: 01 | |
| CCDC28A | P19S | SLGAWRLYLL | 23 | PLGAWRLYLL | 1285 | 4 | 48% | A*02: 01 | |
| CDK5R1 | S96F | FTFAQPPPA | 33 | STFAQPPPA | 551 | 19 | 17% | A*02: 01 | |
| CHST15 | F468C | YLLDWLSVC | 29 | YLLDWLSVF | 23 | 9 | 37% | A*02: 01 | |
| DALRD3 | P427S | GLYSTFPPV | 3 | GLYPTFPPV | 3 | 17 | 24% | A*02: 01 | |
| DDX19B | S129F | LLFSATFEDFV | 42 | SVWKFAQKV | 130 | 19 | 18% | A*02: 01 | |
| ELMSAN1 | S625F | FIAPPVYFNI | 32 | FIAPPVYSNI | 178 | 46 | 31% | A*02: 01 | |
| ESRP2 | P655L | ALASALTSV | 9 | ALASAPTSV | 22 | 36 | 14% | A*02: 01 | |
| FADS2 | S429F | ALLDIIRFL | 8 | ALLDIIRSL | 13 | 1 | 18% | A*02: 01 | |
| FKBP15 | H413Y | SLYPAHPAL | 20 | SLHPAHPAL | 107 | 16 | 20% | A*02: 01 | |
| FLII | A42V | YLPEELVAL | 7 | YLPEELAAL | 8 | 83 | 36% | A*02: 01 | |
| FMO4 | G519R | YLKAWRAPV | 24 | YLKAWGAPV | 12 | 4 | 33% | A*02: 01 | |
| IQGAP3 | P560S | GLDDVSLSV | 6 | GLDDVSLPV | 4 | 10 | 23% | A*02: 01 | |
| ITGB1 | S277F | FTDAGFHFA | 39 | STDAGFHFA | 726 | 260 | 18% | A*02: 01 | |
| B8-2 | RAPGEF2 | S806L | LMFAIISGL | 9 | SMFAIISGL | 10 | 3 | 25% | A*02: 01 |
| RBBP5 | I313F | IIASFSSGV | 37 | FPSPILKV | 11180 | 15 | 17% | A*02: 01 | |
| SEC23IP | P436L | ILDGEMPQV | 9 | IPDGEMPQV | 13477 | 19 | 22% | A*02: 01 | |
| SLC25A30 | P274S | GSWNIIFFV | 20 | GPWNIIFFV | 344 | 11 | 16% | A*02: 01 | |
| SMTN | R665W | KLIWAALREL | 41 | KLIRAALREL | 143 | 23 | 21% | A*02: 01 | |
| SNX19 | L387F | IMFMTPGSFL | 51 | IMLMTPGSFL | 71 | 19 | 29% | A*02: 01 | |
| STRN3 | P406S | ALAFHSVEPV | 19 | ALAFHPVEPV | 23 | 20 | 20% | A*02: 01 | |
| TBL2 | R162C | WLANGDTLCV | 31 | WLANGDTLRV | 33 | 16 | 19% | A*02: 01 | |
| TMCO1 | I65V | FADTLLIVFV | 48 | TLLIVFISV | 24 | 151 | 20% | A*02: 01 | |
| TMEM125 | V126I | VLLSGLVLLI | 24 | VLLSGLVLLV | 10 | 1 | 14% | A*02: 01 | |
| TRAPPC9 | E639K | SLPAKSGLYPV | 44 | SLPAESGLYPV | 41 | 3 | 30% | A*02: 01 | |
| TRIAP1 | P58 L | ILIEGLEFM | 11 | IPIEGLEFM | 11912 | 18 | 13% | A*02: 01 | |
| TRRAP | S702 F | RLPEMGFNV | 16 | RLPEMGSNV | 161 | 1 | 28% | A*02: 01 | |
| UBE3C | P991 L | HLVIKVFWRV | 44 | PVIKVFWRV | 1236 | 20 | 11% | A*02: 01 | |
| URGCP | P310L | GLVEISWFFL | 20 | GLVEISWFFP | 1333 | 11 | 14% | A*02: 01 | |
| USP15 | P800S | KLDLWSLPSV | 5 | KLDLWSLPPV | 4 | 24 | 14% | A*02: 01 | |
| WSB2 | S194L | VLLGHLQWV | 5 | VLSGHLQWV | 10 | 20 | 35% | A*02: 01 | |
| ZNF33A | S288F | FTLSKPHGV | 16 | STLSKPHGV | 183 | 5 | 11% | A*02: 01 | |
| B8-3 | KIAA1429 | D1358E | FLAEHEYGL | 3 | FLAEHDYGL | 3 | 16 | 25% | A*02: 01 |
| KIAA1958 | P400S | KLNKFSVFNI | 37 | KLNKFPVFNI | 39 | 1 | 21% | A*02: 01 | |
| KIF23 | S334F | ITISQLFLV | 11 | ITISQLSLV | 106 | 32 | 27% | A*02: 01 | |
| LIG3 | G76R | YLVFLPRLHV | 35 | YLVFLPGLHV | 29 | 11 | 15% | A*02: 01 | |
| LNPEP | S349F | VQDEFFESV | 21 | VQDEFSESV | 31 | 5 | 20% | A*02: 01 | |
| LPHN3 | H1049Y | KMFHYTAIL | 8 | KMFHHTAIL | 12 | 1 | 27% | A*02: 01 | |
| MAPK13 | G137V | YLVYQMLKV | 9 | YLVYQMLKG | 5484 | 50 | 28% | A*02: 01 | |
| MROH7 | S214F | SLDLDFNPLL | 48 | SLDLDSNPLL | 81 | 1 | 23% | A*02: 01 | |
| MTHFD1L | V418I | VLIAGITPT | 31 | VLVAGITPT | 139 | 31 | 67% | A*02: 01 | |
| MYADM | S68F | FMFTWCFCF | 15 | SMFTWCFCF | 135 | 22 | 15% | A*02: 01 | |
| NID1 | G495E | GIIEWMFAV | 3 | GIIGWMFAV | 4 | 17 | 37% | A*02: 01 | |
| NLRP1 | P614L | ILQEHLIPL | 5 | ILQEHPIPL | 9 | 3 | 10% | A*02: 01 | |
| NPHP4 | P159L | RLYHGTLRAL | 49 | RLYHGTPRAL | 101 | 5 | 18% | A*02: 01 | |
| NUP214 | P1463S | SLPTSFPTL | 41 | PLPTSFPTL | 4229 | 53 | 24% | A*02: 01 | |
| PADI1 | S118F | YLTGVDIFL | 8 | YLTGVDISL | 11 | 1 | 16% | A*02: 01 | |
| PAQR7 | S119F | YLFFSALAHL | 8 | YLSFSALAHL | 15 | 15 | 17% | A*02: 01 | |
| PKP2 | F491 L | TLTENIIIPL | 25 | TLTENIIIPF | 1221 | 5 | 33% | A*02: 01 | |
| PLEKHG4 | S635F | ALPQAFPTV | 19 | ALPQASPTV | 29 | 2 | 25% | A*02: 01 | |
| PTK7 | D463N | MLISENSRFEV | 31 | MLISEDSRFEV | 44 | 103 | 29% | A*02: 01 | |