Table 3.

Cox proportional hazards analysis of the impact of investigative lymphocyte subsets in PT post-NAC, on time to recurrence and overall survival in the entire cohort

	TTR			OS
	n (events)	HR (95 % CI)	P	n (events)	HR (95 % CI)	P
CD8+
Univariable
Low	2 (1)	1.00		2 (2)	1.00
High	91 (44)	1.17 (0.16- 8.49)	0.878	94 (51)	0.71 (0.17-2.94)	0.641
Multivariable
Low	1 (1)	1.00		1 (2)	1.00
High	89 (44)	161627.27 (0.00-2.24E+290)	0.971	92 (51)	2.29 (0.26-20.23)	0.457
FoxP3+
Univariable
Low	85 (38)	1.00		87 (43)	1.00
High	7 (6)	2.77 (1.16-6.63)	0.022	7 (6)	3.18 (1.33-7.61)	0.009
Multivariable
Low	82 (38)	1.00		84 (43)	1.00
High	7 (6)	2.40 (0.87-6.58)	0.090	7 (6)	2.86 (1.03-7.90)	0.043
CD20+
Univariable
Low	68 (31)	1.00		70 (34)	1.00
High	23 (14)	1.53 (0.81-2.88)	0.188	24 (18)	1.59 (0.90-2.82)	0.112
Multivariable
Low	65 (31)	1.00		67 (34)	1.00
High	23 (14)	1.45 (0.71-2.95)	0.303	24 (18)	1.49 (0.80-2.78)	0.213

Classification and regression tree-derived prognostic cutoffs are used to define ”low” and ”high” in all analyses. TTR = time to recurrence, OS = overall survival, HR = hazard ratio. Multivariable analysis includes age, sex, location, T-stage (T1-2 vs T3-4), N-stage (N0 vs N+), M-stage (M0 vs M1), differentiation grade (high vs low), resection margin (R0, R1, R2), Laurén (intestinal vs diffused/mixed type).