Figure 3.

ΔPSMα Aggregate2 is composed of an 8-mer oligomer. (a) Results of analytical ultracentrifugation on ΔPSMα Aggregate2. The curve is the sedimentation coefficient distribution plot. The molecular weight was calculated using Sedfit 11.7 software. (b) Static light scattering (SLS) study of ΔPSMα Aggregate2. The molecular weight was calculated using ASTRA 6.1, and is shown beside the peak. (c) ΔPSMα Aggregate2 is analyzed in the native MALDI-TOF-MS with a mass-to-charge ratio (m/z). The estimated number of monomers and the molecular mass are shown above the peaks. (d) Tricine-SDS-PAGE shows the cross-link assay results of ΔPSMα Aggregate2 enhanced by the crosslinker BS3. The number of monomers are shown in the images. All the experiments were repeated independently three times with similar results