Figure 4.

Tricine-SDS-PAGE analysis of the components of co-immunoprecipitation (Co-IP). (a-b) The images of Tricine-SDS-PAGE stained by Coomassie Blue. The upper two bands in the gel are antibodies, the lower band consists of Co-IP protein products. The band components have been digested in-gel and identified by LC-MS/MS. (a-b) correspond to different samples: (a) is Aggregate2 from MW2 and its mutants, (b) is the supernatant of MW2 and its mutants. Perhaps due to the affinity of the PSMα antibody, we could not obtain a sufficient amount of product in supernatant Co-IP assays (b). All the experiments were repeated independently three times with similar results