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. 2021 May 24;12(1):1418–1437. doi: 10.1080/21505594.2021.1928395

Figure 9.

Figure 9.

Impact of adding dfδ-toxin oligomer on the structure of static S. aureus biofilms. Static biofilms were grown in eight-well chambered coverglass plates for 48 h, with adding dfδ-toxin oligomer (a, b) before the formation of the biofilm (0 h) or (c, d) after the formation of the biofilm (36 h). (a, c) Three-dimensional confocal laser scanning microscopy (CLSM) images of biofilms. Extensions and scale are the same in every image (total x extension: 160 μm; total y extension: 160 μm). (b, d) Biofilm parameters were measured in at least 5 randomly chosen biofilm CLSM images of the same extension on a Fluoview 1000. Data represent means ± SD. ANOVA was used to determine statistical significance followed by Dunnett’s multiple comparison test. All the experiments were repeated independently three times with similar results