Table 1.

Affinity of monoclonal antibodies (biolayer interferometry)

Antibody variant	KD (nM)	ka (M−1.s−1)	kd (s−1)	Neutralization IC50 (µg/ml)
m396 parental	nd	nd	nd	nd
m396-C4	13.0	8.13 x 104	1.06 x 10−3	0.34
m396-B10	7.1	1.01 x 105	7.18 x 10−4	0.16
CR3022 parental	99.2	7.19 x 104	7.13 x 10−3	nd
CR3022-B11	131.0	1.02 x 105	1.33 x 10−2	nd
CR3022-G11	93.6	2.41 x 105	2.26 x 10−2	nd
CR3022-B6	261.0	7.62 x 104	1.99 x 10−2	4.43
CR3022-M	188.0	7.94 x 104	1.49 x 10−2	0.35
80R parental	nd	nd	nd	nd
80R-A2	61.0	6.58 x 104	4.01 x 10−3	17.83
CR3014 parental	nd	nd	nd	nd
CR3014-D1	50.8	7.95 x 104	4.04 x 10−3	>100
CR3014-C8	61.1	5.84 x 104	3.57 x 10−3	nd

nd: no binding/neutralization detected