Table 1.
Glycosylation of the secreted form of PD-L1 (Phe19-Thr239) produced by human HEK293, mouse NS0, and human MDA-MB-231 cells.
| Origin | Site | SO | GP# | Glycan | Type | % |
|---|---|---|---|---|---|---|
| HEK293 (human) | N35 | >99% | 44 | HexNAc(6)Hex(4)SA(1) | CL | 13 |
| HexNAc(6)Hex(5) | CL | 8 | ||||
| HexNAc(6)Hex(4)Fuc(1) | CL | 6 | ||||
| N192 | >99% | 75 | HexNAc(4)Hex(4) | CL | 15 | |
| HexNAc(4)Hex(4)Fuc(1) | CL | 13 | ||||
| HexNAc(4)Hex(5) | CL | 12 | ||||
| N200 | >81% | 61 | HexNAc(5)Hex(4)Fuc(1)SA(2) | CL | 19 | |
| HexNAc(5)Hex(4)SA(2) | C | 18 | ||||
| HexNAc(5)Hex(4)Fuc(2) | C | 5 | ||||
| N219 | <0.1% | 0 | NQ | n/a | n/a | |
| NS0 (mouse) | N35 | 98% | 7 | HexNAc(5)Hex(9)Fuc(1) | GG | 43 |
| HexNAc(5)Hex(8)Fuc(1) | GG | 30 | ||||
| HexNAc(9)Hex(4) | CL | 14 | ||||
| N192 | >99% | 19 | HexNAc(2)Hex(5) | HM | 47 | |
| HexNAc(2)Hex(7) | HM | 32 | ||||
| HexNAc(2)Hex(6) | HM | 17 | ||||
| N200 | 97% | 17 | HexNAc(5)Hex(9)Fuc(1) | GG | 67 | |
| HexNAc(4)Hex(7)Fuc(1) | GG | 5 | ||||
| HexNAc(5)Hex(8)Fuc(1) | GG | 5 | ||||
| N219 | >99% | 34 | HexNAc(8)Hex(8) | CP | 59 | |
| HexNAc(8)Hex(7) | CP | 22 | ||||
| HexNAc(8)Hex(6) | CP | 12 | ||||
| MDA-MB-231 (human) | N35 | 91% | 24 | HexNAc(4)Hex(6)Fuc(1) | H | 26 |
| HexNAc(4)Hex(6)Fuc(2) | H | 23 | ||||
| HexNAc(2)Hex(5) | HM | 10 | ||||
| N192 | >99% | 34 | HexNAc(2)Hex(9) | HM | 25 | |
| HexNAc(3)Hex(5)SA(1) | H | 16 | ||||
| HexNAc(2)Hex(6) | HM | 12 | ||||
| N200 | >99% | 30 | HexNAc(4)Hex(5)Fuc(1) | C | 43 | |
| HexNAc(4)Hex(4)Fuc(1) | C | 12 | ||||
| HexNAc(4)Hex(3)Fuc(1) | PM | 8 | ||||
| N219 | <1% | 9 | HexNAc(5)Hex(5) | C | 33 | |
| HexNAc(4)Hex(5)Fuc(3) | C | 18 | ||||
| HexNAc(2)Hex(5) | HM | 17 |
Glycoforms were analyzed on four glycopeptides: DLYVVEYGSNMTIECK (N35), LFNVTSTLR (N192), INTTTNEIFYCTFR (N200), and LDPEENHTAELVIPELPLAHPPNER (N219). Abbreviations: HexNAc, N-acetylhexosamine; Hex, Hexose; Fuc, Fucose; SA, sialic acid; SO, site occupancy; GP#, number of identified glycopeptides; NQ, not quantifiable. Three most abundant glycoforms for each site are listed. Types of glycan structure: HM, high mannose; H, hybrid; C, complex; CL, complex glycan containing LacdiNAc; CP, complex glycan containing polyLacNAc; GG, complex glycan containing Gal-Gal (Galili); PM, paucimannose.