Table 2.
Glycosylation of the four glycopeptides of PD-L1 isolated from whole cell lysate and enriched cell-surface fraction of MDA-MB-231 cells.
| Total |
Membrane |
|||||||
|---|---|---|---|---|---|---|---|---|
| Site | SO (GP#) |
Glycan | Type | % | SO (GP#) |
Glycan | Type | % |
| N35 | >99% (4) |
HexNAc(4)Hex(5)Fuc(1)SA(1) | C | 43 | >99% (1) |
HexNAc(4)Hex(5)Fuc(3) | C | 100 |
| HexNAc(2)Hex(9) | HM | 34 | ||||||
| HexNAc(4)Hex(5)Fuc(3) | C | 18 | ||||||
| HexNAc(2)Hex(9) | HM | 5 | ||||||
| N192 | >99% (6) |
HexNAc(2)Hex(7) | HM | 46 | >99% (5) |
HexNAc(2)Hex(7) | HM | 47 |
| HexNAc(2)Hex(8) | HM | 25 | HexNAc(2)Hex(8) | HM | 42 | |||
| HexNAc(2)Hex(9) | HM | 14 | HexNAc(2)Hex(9) | HM | 8 | |||
| HexNAc(2)Hex(6) | HM | 7 | HexNAc(2)Hex(5) | HM | 2 | |||
| HexNAc(2)Hex(5) | HM | 2 | HexNAc(2)Hex(6) | HM | 1 | |||
| HexNAc(2)Hex(4) | HM | 1 | ||||||
| N200 | >99% (6) |
HexNAc(2)Hex(9) | HM | 38 | >99% (4) |
HexNAc(4)Hex(5)Fuc(1)SA(2) | C | 40 |
| HexNAc(2)Hex(8) | HM | 32 | (4) HexNAc(4)Hex(5)Fuc(1)SA(1) | C | 26 | |||
| HexNAc(4)Hex(5)Fuc(1)SA(1) | C | 12 | HexNAc(6)Hex(7)Fuc(1)SA(3) | C | 20 | |||
| HexNAc(4)Hex(5)Fuc(1)SA(2) | C | 6 | HexNAc(7)Hex(8)SA(1) | CP | 14 | |||
| HexNAc(2)Hex(7) | HM | 6 | ||||||
| HexNAc(5)Hex(6)Fuc(3)SA(1) | C | 4 | ||||||
| N219 | 92% (14) |
HexNAc(2)Hex(9) | HM | 26 | 80% (10) |
HexNAc(5)Hex(6)Fuc(1)SA(2) | CP | 22 |
| HexNAc(2)Hex(8) | HM | 11 | HexNAc(7)Hex(8)SA(1) | CP | 19 | |||
| HexNAc(5)Hex(6)Fuc(1)SA(2) | CP | 8 | HexNAc(7)Hex(8)Fuc(1)SA(2) | CP | 18 | |||
| HexNAc(7)Hex(8)Fuc(1)SA(2) | CP | 8 | HexNAc(7)Hex(8)Fuc(1)SA(3) | CP | 16 | |||
| HexNAc(2)Hex(7) | HM | 7 | HexNAc(4)Hex(5)Fuc(1)SA(2) | C | 8 | |||
| HexNAc(5)Hex(6)Fuc(1)SA(1) | CP | 7 | HexNAc(5)Hex(6)Fuc(3) | CP | 5 | |||
| HexNAc(2)Hex(6) | HM | 6 | HexNAc(4)Hex(5)Fuc(1)SA(1) | C | 5 | |||
| HexNAc(6)Hex(7)Fuc(1)SA(2) | CP | 5 | HexNAc(5)Hex(6)Fuc(1)SA(1) | CP | 4 | |||
| HexNAc(7)Hex(8)SA(1) | CP | 4 | HexNAc(6)Hex(7)Fuc(1)SA(4) | C | 3 | |||
| HexNAc(4)Hex(5)Fuc(1)SA(2) | C | 3 | HexNAc(6)Hex(7)Fuc(1)SA(3) | CP | 1 | |||
| HexNAc(7)Hex(8)Fuc(1)SA(4) | CP | 3 | ||||||
| HexNAc(7)Hex(8)Fuc(1)SA(3) | CP | 2 | ||||||
| HexNAc(6)Hex(7)Fuc(1)SA(4) | C | 2 | ||||||
| HexNAc(2)Hex(5) | HM | 1 | ||||||
Abbreviations: HexNAc, N-acetylhexosamine; Hex, Hexose; Fuc, Fucose; SA, sialic acid; SO, site occupancy, GP#, number of identified glycopeptides. Types of glycan structure: HM, high mannose; C, complex; CP, complex glycan containing polyLacNAc