Table 1.
Summary of growth Inhibition and bacterial protection phenotypes in response to treatment or pre-treatment with OMVs
| Growth inhibition?a | Bacterial protection?a | ||||
|---|---|---|---|---|---|
| Treatment | Pst C | Pst M | Pf C | Pf M | Pst M |
| Sonication | Y | Y | Y | Y | Y |
| Salt strip (NaCl) | Nb | Nb | Yb | Yb | – |
| UV (30 min) | N | N | N | N | – |
| Frozen in LN2 and lyophilized | Y | N | Y | Y | – |
| Boiled (100°C, 2 hr) | N | N | N | N | Y |
| Proteinase K (100 μM, 1 h, 37°C) | N | N | N | N | Y/N |
| Combined (polymyxin, sonicate, benzonase, Proteinase K, Tween 20, boil) | N | N | Y/Nc | N | Y/Nc |
| Organic extraction (MeOH and DCM) | Y/Nd | – | – | – | – |
| Size exclusion on extraction pellet | Y/Ne | – | – | – | – |
See also Figures 2E, 2F, S5, and S6.
Seedlings (for growth inhibition) or 3-week-old plants (for bacterial protection) were treated with OMVs from the indicated strain and condition. OMVs were treated before application with the indicated biochemical or physical stressors. C: OMVs isolated from complete media; M: OMVs isolated from minimal media; Y: activity retained in OMVs; N: activity reduced to level of buffer-treated control; Y/N: activity reduced to a level between OMVs and buffer-treated control. –: activity not tested for these conditions.
Activity was also assessed for post-treatment supernatants and activity was detected in supernatant.
Activity was <5× Pst OMVs and greater than buffer control, but not statistically different from the negative control with treatments alone.
Activity was reduced compared to unfractionated 5× Pst OMVs but retained in both fractions.
Activity was reduced compared to unfractionated 5× Pst OMV extraction pellet, but only in the fraction containing components larger than 10 kDa.