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. 2021 May 18;22(10):5314. doi: 10.3390/ijms22105314

Figure 5.

Figure 5

(A) Western blot analyses of Rubisco large (LSU) and small (SSU) subunit, as well as (B) SUMO conjugates extracted from leaves of WT BIG8 and mutant BIG8-1 plants under well-watered (Day 0) and after exposure to water deficit stress (Day 7, 14, 21 PWS). Each lane contains 20 μg protein. The proteins were transferred to a nitrocellulose membrane and probed with polyclonal IgG raised against LSU and SSU (1:7000 dilution); while in the case of the SUMO conjugates with a monoclonal IgG raised against SUMO1 (1:10,000 dilution). M1–M3 represents result from three independent mutant plants.