Skip to main content
. 2021 May 18;22(10):5314. doi: 10.3390/ijms22105314

Figure 6.

Figure 6

Proteolytic activity of WT BIG8 and mutant BIG8-1 plants under well-watered (Day 0) and after exposure to water deficit stress. Gradient zymograms (5–15%) were loaded with 35 μg protein per lane. An incubation step with 0.1 mM cysteine protease inhibitor (E-64) was included to allow for the identification of cysteine proteases (CPs) during (A) Day 0, (B) Day 7, (C) Day 14 and (D) Day 21. + PI indicates the lanes with the protein that was pre-treated with 0.1 mM cysteine protease inhibitor (E-64), − PI indicates no incubation with E-64. Arrows indicate absence of bands due to treatment with the 0.1 mM E-64. Presented data are representative for two independent experiments.