Table 1.

Isolated leaf free amino acids from leave material of the BIG8 (WT) and BIG8-1 (Mutant) lines under well-watered (Day 0) and after induction of water deficit stress (Days 7, 14 and 21).

Genotype	Treatment	Free Amino Acid (FAA) Level
		His	Ser	Arg	Gly	Asp	Glu	Thr	Ala	Pro	Lys	Tyr	Met	Val	Ile	Leu	Phe
BIG8 (WT) *	Day 0	0.10	0.33	0.32	0.31	0.55	0.59	0.24	0.39	0.29	0.39	0.21	0.54	0.31	0.18	0.42	0.34
	Day 7	n.d	0.20	n.d	0.27	n.d	0.56	0.31	0.42	0.88	0.50	0.14	2.54	0.14	0.33	0.59	1.65
BIG8-1 (Mutant)	Day 0	0.16	0.35	0.45	0.34	0.84	0.69	0.32	0.37	0.33	0.50	0.24	0.43	0.36	0.19	0.80	0.59
	Day 7	0.10	0.14	n.d	0.12	0.24	0.33	0.06	0.13	0.31	0.18	0.08	0.75	0.08	0.06	n.d	0.42
	Day 14	0.14	0.26	0.27	0.30	0.80	0.68	0.22	0.36	0.88	0.34	0.17	0.54	0.36	0.20	0.66	0.41
	Day 21	0.31	0.41	0.59	0.57	1.71	2.37	0.32	0.51	4.60	0.38	0.39	0.46	0.77	0.43	0.76	0.84

* Measurements could be only taken for the WT until Day 7, as it suffered irreversible damage and was dead by Day 9 (n = 3).