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. 2021 May 18;12(5):770. doi: 10.3390/genes12050770

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(Over)expression of SprF1 antitoxin decreases the level of gene transcripts expressing proteins identified as downregulated in the intracellular proteome. (A) RT-qPCR analysis of mRNAs isolated from S. aureus N315 (WT pCN35), sprG1/sprF1 deletion mutant (ΔΔ pCN35), and the sprG1/sprF1 deletion mutant with the SprF1 antitoxin expressing (ΔΔ pCN35ΩsprF1) plasmid. * and **, denote statistical significance at p < 0.05 and p < 0.01 level, respectively. (B) A model of in silico predicted interactions between the gene transcript (ppiB) for peptidyl-prolyl cis-trans isomerase and the SprF1 antitoxin (sRNA). (C) EMSA of radioactively labeled SprF1 (SprF1 *) with mRNA for ppiB. SprG1/SprF1 * complex serves as a positive control.