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. 2021 May 18;12(5):770. doi: 10.3390/genes12050770

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of the SprG1-encoded peptides during the S. aureus stationary growth phase does not induce massive cell leakages. (A) RT-qPCR analysis of the transcript level for SprG1 toxin in S. aureus N315 (statistical significance at p < 0.01 level). (B) Western blot analysis with anti-GPF antibodies of cell lysates and spent growth medium of S. aureus N315 (WT) and the sprG1/sprF1 deletion mutant (ΔΔ) transformed with a GFP-expressing plasmid (pALCP2G). (C) Western blot with anti-Flag antibodies and SDS-PAGE of total cell lysates (intracellular proteins; upper panels) and culture medium (extracellular proteins; lower panels) of S. aureus N315 (WT pCN35), the sprG1/sprF1 deletion mutant (ΔΔ pCN35), SprF1 antitoxin (over)expressing (N315ΔΔ pCN35ΩsprF1), and Flagged SprG1 (over)expressing (N315ΔΔ pCN35ΩsprG1-Flag) strains grown to logarithmic and stationary phases.