. Author manuscript; available in PMC: 2021 May 27.

Published in final edited form as: Nature. 2009 Apr 8;459(7244):270–273. doi: 10.1038/nature07937

Table 3.

Effect of PfCRT position 76 mutations on chemosensitization of quinine by T3.5 and verapamil in P. falciparum.

Compound	IC₅₀^* (nM) P. falciparum
Compound	106/1^K76^**	106/1^76T^***	106/1^76N^***	106/1^76I^***
Verapamil alone	25800 ± 128	1779 ± 105	632 ± 78	8630 ± 312
T3.5 alone	93.6 ± 12	86.9 ± 8.5	134 ± 15	100.8 ± 18
Chloroquine alone	15.9 ± 1.4	188 ± 2.3	147 ± 6.8	158 ± 4.4
Chloroquine + 500 nM verapamil	17.2 ± 1.6	51.2 ± 3.2	39.9 ± 5.4	47.7 ± 5.6
Chloroquine + 25 nM T3.5	14.5 ± 2.1	67.8 ± 9.8	74.3 ± 6.8	58.5 ± 6.1
Quinine alone	112 ± 18	204 ± 19	186 ± 21	20.8 ± 2.1
Quinine + 500 nM verapamil	118 ± 10	71.4 ± 8.9	53.5 ± 4.6	32.1 ± 2.4
Quinine + 25 nM T3.5	55.6 ± 4.0	56.7 ± 5.3	71.2 ± 6.8	5.5 ± 0.8

Values are the means ± standard errors of the mean (S.E.M.) from three independent experiments, each in quadruplicate, utilizing the MSF assay with 0.2% parasitemia and 2% hematocrit.

^**

Sudan 106/1^K76 is known to have 7 of the 8 mutations in the pfcrt gene necessary for chloroquine resistance.

^***

Mutant line selected with chloroquine pressure from 106/1^K76.