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. 2021 May 12;12:639049. doi: 10.3389/fimmu.2021.639049

Figure 1.

Figure 1

BAFF priming induced a proinflammatory memory response of BV2 cells to LPS and BAFF itself. (A) Graphical outline of the in vitro methods. BV2 cells or primary mouse microglial cells were incubated for 24 h with vehicle or BAFF (defined as ‘BAFF priming’), and after a 5-day rest period, microglial cells were restimulated with 40 ng/ml BAFF (BAFF restimulation) or 100 ng/ml LPS (LPS restimulation) for 24 h. In (B–D), BV2 cells were primed with different doses of BAFF (2.5, 5, 10 ng/ml) or vehicle for 24 h, rested for 5 days, on day 6 treated with LPS restimulation for 24 h. TNF-α (B), IL-6 (C), and IL-1β (D) were measured in the supernatants by ELISA on day 7(n = 12). On day 6, BV2 cells were restimulated with BAFF instead of LPS. On day 7, TNF-α (E), IL-6 (F) and IL-1β (G) were measured in the supernatants (n = 12). Data are means ± SED; Kruskal-Wallis test with Turkey post hoc test; *p < 0.05, **p < 0.01, and ***p < 0.001.