Figure 3.

Inhibition of glycolysis by 2-DG blocked proinflammatory cytokines secretion of BAFF-training BV2 cells. BV2 cells were pretreated with 1mM 2-DG for 1 h, then primed with vehicle or BAFF for 24 h. After a rested of 5 days, cells were restimulated with LPS or BAFF for 24 h. The amounts of TNF-α (A) and IL-6 (B) were measured in the culture supernatants and the cytotoxicity of 2-DG was measured by MTT (C) on day 7 upon LPS restimulation (n = 12). The secretion of TNF-α (D) and IL-6 (E) and the cytotoxicity of 2-DG (F) were measured on day 7 upon BAFF restimulation (n = 12). The Data are mean ± SEM; Kruskal-Wallis test with Turkey post hoc test; ***p < 0.001. ns: no significance between groups.