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. 2021 May 12;12:639049. doi: 10.3389/fimmu.2021.639049

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Copyright © 2021 Wang, Yang, Hou, Xu, Yun, Qin and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BAFF-induced metabolic reprogramming was blocked by rapamycin in BV2 cells. (A) On day 0, BV2 cells were pretreated with 100 nM rapamycin for 1 h and then treated with vehicle or BAFF (10 ng/ml) for 24 h, rested for 5 days. BV2 cells were lysed on day 6. Whole-cell extracts were subjected to western blot for the expression level of mTOR, p-mTOR, and HIF-1α. Densitometric quantification of p-mTOR/mTOR (B) and HIF-1α/tubulin (C) in (A) (n = 9). (D) Lactate was measured in the culture supernatants on day 6 (n = 12). (E) The ratio of NAD+/NADH was detected in whole-cell lysates on day 6 (n = 9). Data are mean ± SEM; Kruskal-Wallis test with Turkey post hoc test; **p < 0.01, and ***p < 0.001.