Table 1.
Ingredient composition and proximate analysis (as-is basis) of a grain-free pet food formula produced to evaluate the effect of Bacillus coagulans in an extruded dog kibble application
| Formulation | Amount |
|---|---|
| Ingredients | |
| Chicken meal, % | 34.64 |
| Peas, dehydrated, % | 20.00 |
| Sweet potatoes, flaked, % | 20.00 |
| Chicken fat, % | 8.50 |
| Tapioca flour, % | 5.00 |
| Pea protein, % | 5.00 |
| Beet pulp, % | 3.00 |
| Digest flavoring, % | 1.00 |
| Potassium chloride, % | 0.50 |
| Salt, % | 0.50 |
| Dicalcium phosphate, % | 0.50 |
| Titanium dioxide1, % | 0.40 |
| dl-Methionine, % | 0.25 |
| Choline chloride, % | 0.20 |
| Fish oil, % | 0.20 |
| Vitamin premix2, % | 0.15 |
| Trace mineral premix3, % | 0.10 |
| Natural antioxidant, % | 0.07 |
| B. coagulans (15B CFU·g−1) | * |
| Analyzed nutrient composition | |
| Moisture, % | 4.92 |
| Crude protein, % | 34.90 |
| Crude fat, % | 15.60 |
| Crude fiber, % | 3.28 |
| Ash, % | 9.21 |
| Nitrogen-free extract (NFE), % | 32.09 |
| ME4, kcal·kg−1 | 3,671 |
1Food-grade TiO2 was used as an indigestible marker for digestibility calculations.
2Vitamin premix: pea fiber, calcium carbonate, vitamin E supplement, niacin supplement, thiamine mononitrate, d-calcium pantothenate, vitamin A supplement, sunflower oil, pyridoxine hydrochloride, riboflavin supplement, vitamin D3 supplement, biotin, vitamin B12 supplement, and folic acid.
3Trace mineral premix: zinc proteinate, calcium carbonate, zinc sulfate, iron proteinate, ferrous sulfate, copper proteinate, copper sulfate, manganese proteinate, sunflower oil, sodium selenite, manganous oxide, calcium iodate, and ethylenediamine dihydroiodide.
4ME of diets was calculated using modified Atwater factors of 3.5, 3.5, and 8.5 kcal/g for energy from crude protein, NFE, and crude fat, respectively (NRC, 2006).
*Each experimental diet contained differing levels of B. coagulans applied as reported in Table 2.