Table 2.
Application method and concentration of Bacillus coagulans in five experimental diet treatments (as-is basis)
| Treatment1 | |||||
|---|---|---|---|---|---|
| Bacillus coagulans treatment | CON | PEX | PCL | PCM | PCH |
| Application method | None | Base ration | Coating | Coating | Coating |
| Formula inclusion2, % | 0.00 | 0.03 | 0.0002 | 0.002 | 0.02 |
| Analyzed CFU·g−1 in ration3 | 0.00 | 4.58 × 106 | 0.00 | 0.00 | 0.00 |
| Analyzed CFU·g−1 in diet4 | 0.00 | 1.06 × 104 | 5.92 × 104 | 6.86 × 105 | 6.84 × 106 |
| Dose (CFU·dog−1·d−1)5 | 0.00 | 2.12 × 106 | 1.18 × 107 | 1.37 × 108 | 1.37 × 109 |
1CON, control; PEX, DFM applied before extrusion; DFM applied as coating at low dose; PCM, DFM applied as coating at moderate dose; PCH, DFM applied as coating at high dose.
2Formula inclusion as percent of batch weight with B. coagulans added as a powder with 15 billion CFU·g−1 (Kerry, Inc., Beloit, WI, USA).
3Bacillus coagulans CFU counts analyzed in base ration before extrusion.
4Bacillus coagulans CFU counts analyzed in extruded, dried, and coated diets at time of feeding.
5Based on an expected average daily food intake of 200 g·dog−1·d−1