Table 3.

Hemagglutination inhibition (HI) titers obtained for individual sera taken between December 2016 and May 2017 in non-mixed herd #1 (ten sows) and mixed herd #2 (two backyard pigs).

Herd ID	Sampling Date	Pig ID	ELISA S/P 2		HI Titres Obtained with Influenza A Virus Strains 1 of Different Origins, Subtypes and Lineages
					Swine IAV					Avian IAV						Human IAV
				HA Clade	1C.2.1	1A.3.3.2	1B.1	1B.1.2.3	H3	H5-2.3.4.4		H5-Others		Hx(N8)		H3
				Subtype	H1avN1	H1N1pdm	H1huN2	H1huN2 Δ146–147	H3N2	H5N8	H5N5	H5N9	H5N3	H3N8	H6N8	H3N2
#1	01 Feb	#1	21.8		10	<10	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#2	4.7		80	80	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#3	13.0		20	<10	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#4	18.8		20	40	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#5	22.3		<10	<10	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#6	5.1		20	20	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#7	5.9		40	80	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#8	18.2		<10	20	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#9	5.4		40	160	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
		#10	5.4		10	10	<10	<10	<10	<10	nd	<10	<10	nd	nd	nd
#2	23 Dec	#1	10.2		<10	<10	<10	<10	<10	40	20	<10	<10	<10	<10	10
	23 Dec	#2	58.5		<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	20
	01 Feb	#1	24.8		<10	<10	<10	<10	<10	40	20	<10	<10	<10	<10	<10
	01 Feb	#2	9.1		<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	20
	31 May	nd	41.7		<10	<10	<10	nd	<10	<10	nd	<10	<10	nd	nd	10

Results above the test positive threshold (20) are indicated in bold. Results obtained in ELISA are indicated as S/P values for information. ¹ Strains used as antigens are given in the main text and in Table 2. ² S/P is result of competition ELISA obtained by the following calculation = OD sample/OD negative control*100. If the result is ≤45%, the sample is interpreted positive; comprised between 45 and 50% it is doubtful; ≥50% it is negative. ID: identifier; nd: not determined.